Changes in phosphorylation of 50 and 53 kDa soluble proteins in graviresponding oat (Avena sativa) shoots

The present work indicates that phosphorylation of a 50 kDa soluble protein is involved in the gravitropic response in graviresponsive pulvini of oat (Avena sativa) stems. This 50 kDa protein shows a differential pattern of phosphorylation between lower and upper halves of pulvini both in vivo and i...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 54(2003), 384 vom: 02. März, Seite 1013-22
1. Verfasser: Chang, Soo Chul (VerfasserIn)
Weitere Verfasser: Cho, Myeon Haeng, Kim, Seong-Ki, Lee, June Seung, Kirakosyan, Ara, Kaufman, Peter B
Format: Aufsatz
Sprache:English
Veröffentlicht: 2003
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Non-programmatic Plant Proteins lanthanum chloride 04M8624OXV Lanthanum 6I3K30563S Cycloheximide 98600C0908
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520 |a The present work indicates that phosphorylation of a 50 kDa soluble protein is involved in the gravitropic response in graviresponsive pulvini of oat (Avena sativa) stems. This 50 kDa protein shows a differential pattern of phosphorylation between lower and upper halves of pulvini both in vivo and in vitro. The differential phosphorylation of this protein is detected only when stem segments are gravistimulated for short and long time periods. The differential phosphorylation of the 50 kDa protein occurs as early as 5 min after the initiation of gravistimulation. This corresponds closely to the presentation time of 5.2 min. This differential phosphorylation pattern was changed by treatments with cycloheximide, implying that a newly-synthesized protein is involved in the differential phosphorylation during the gravitropic response. An autophosphorylation experiment shows that the 50 kDa protein has kinase activity. The phosphorylation patterns of a 53 kDa protein were similar to those of the 50 kDa protein, but were only expressed in vitro. These findings indicate that the differential phosphorylation of the 50 (and 53 kDa) soluble proteins in graviresponding oat shoots may be an important component of the gravity signal transduction pathway 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
650 4 |a Non-programmatic 
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700 1 |a Cho, Myeon Haeng  |e verfasserin  |4 aut 
700 1 |a Kim, Seong-Ki  |e verfasserin  |4 aut 
700 1 |a Lee, June Seung  |e verfasserin  |4 aut 
700 1 |a Kirakosyan, Ara  |e verfasserin  |4 aut 
700 1 |a Kaufman, Peter B  |e verfasserin  |4 aut 
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