Multiple signalling pathways mediate fungal elicitor-induced beta-thujaplicin biosynthesis in Cupressus lusitanica cell cultures

Multiple signalling pathways mediate fungal elicitor-induced beta-thujaplicin biosynthesis in Cupressus lusitanica cell cultures

The biosynthesis of a phytoalexin, beta-thujaplicin, in Cupressus lusitanica cell cultures can be stimulated by a yeast elicitor, H(2)O(2), or methyl jasmonate. Lipoxygenase activity was also stimulated by these treatments, suggesting that the oxidative burst and jasmonate pathway may mediate the el...

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Détails bibliographiques
Publié dans:Journal of experimental botany. - 1985. - 54(2003), 383 vom: 01. Feb., Seite 647-56
Auteur principal: Zhao, Jian (Auteur)
Autres auteurs: Sakai, Kokki
Format: Article
Langue:English
Publié: 2003
Accès à la collection:Journal of experimental botany
Sujets:Journal Article Research Support, Non-U.S. Gov't Acetates Cyclopentanes Intercellular Signaling Peptides and Proteins Monoterpenes Oxylipins Peptides Plant Growth Regulators Wasp Venoms plus... lanthanum chloride 04M8624OXV Calcimycin 37H9VM9WZL Egtazic Acid 526U7A2651 Suramin 6032D45BEM Lanthanum 6I3K30563S mastoparan 72093-21-1 Tropolone 7L6DL16P1T methyl jasmonate 900N171A0F Cholera Toxin 9012-63-9 Hydrogen Peroxide BBX060AN9V Verapamil CJ0O37KU29 Lipoxygenase EC 1.13.11.12 GTP Phosphohydrolases EC 3.6.1.- GTP-Binding Proteins Calcium SY7Q814VUP beta-thujaplicin U5335D6EBI
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245 1 0 |a Multiple signalling pathways mediate fungal elicitor-induced beta-thujaplicin biosynthesis in Cupressus lusitanica cell cultures 
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500 |a Date Completed 16.06.2003 
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500 |a ErratumIn: J Exp Bot. 2003 Sep;54(390):2195 
500 |a Citation Status MEDLINE 
520 |a The biosynthesis of a phytoalexin, beta-thujaplicin, in Cupressus lusitanica cell cultures can be stimulated by a yeast elicitor, H(2)O(2), or methyl jasmonate. Lipoxygenase activity was also stimulated by these treatments, suggesting that the oxidative burst and jasmonate pathway may mediate the elicitor-induced accumulation of beta-thujaplicin. The elicitor signalling pathway involved in beta-thujaplicin induction was further investigated using pharmacological and biochemical approaches. Treatment of the cells with calcium ionophore A23187 alone stimulated the production of beta-thujaplicin. A23187 also enhanced the elicitor-induced production of beta-thujaplicin. EGTA, LaCl(3), and verapamil pretreatments partially blocked A23187- or yeast elicitor-induced accumulation of beta-thujaplicin. These results suggest that Ca(2+) influx is required for elicitor-induced production of beta-thujaplicin. Treatment of cell cultures with mastoparan, melittin or cholera toxin alone or in combination with the elicitor stimulated the production of beta-thujaplicin or enhanced the elicitor-induced production of beta-thujaplicin. The G-protein inhibitor suramin inhibited the elicitor-induced production of beta-thujaplicin, suggesting that receptor-coupled G-proteins are likely to be involved in the elicitor-induced biosynthesis of beta-thujaplicin. Indeed, both GTP-binding activity and GTPase activity of the plasma membrane were stimulated by elicitor, and suramin and cholera toxin affected G-protein activities. In addition, all inhibitors of G-proteins and Ca(2+) flux suppressed elicitor-induced increases in lipoxygenase activity whereas activators of G-proteins and the Ca(2+) signalling pathway increased lipoxygenase activity. These observations suggest that Ca(2+) and G-proteins may mediate elicitor signals to the jasmonate pathway, and the jasmonate signalling pathway may then lead to the production of beta-thujaplicin 
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