Creating randomized amino acid libraries with the QuikChange Multi Site-Directed Mutagenesis Kit

Creating randomized amino acid libraries with the QuikChange Multi Site-Directed Mutagenesis Kit

The QuikChange Multi Site-Directed Mutagenesis Kit is a simple and efficient method for introducing point mutations at up to five sites simultaneously in plasmid DNA templates. Here we used the QuikChange Multi kit with degenerate (one codon) primers to introduce all possible amino acids at selected...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 33(2002), 5 vom: 21. Nov., Seite 1158-60, 1162, 1164-5
1. Verfasser: Hogrefe, Holly H (VerfasserIn)
Weitere Verfasser: Cline, Janice, Youngblood, Geri L, Allen, Ronda M
Format: Aufsatz
Sprache:English
Veröffentlicht: 2002
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Evaluation Study Journal Article Codon, Terminator DNA Primers DNA, Recombinant Recombinant Proteins Pfu DNA polymerase EC 2.7.7.- DNA-Directed DNA Polymerase EC 2.7.7.7 mehr... beta-Galactosidase EC 3.2.1.23