A pH-sensitive fluor, CypHer 5, used to monitor agonist-induced G protein-coupled receptor internalization in live cells

A pH-sensitive fluor, CypHer 5, used to monitor agonist-induced G protein-coupled receptor internalization in live cells

G protein-coupled receptors (GPCRs) are the largest family of proteins involved in transmembrane signal transduction and are actively studied because of their suitability as therapeutic small-molecule drug targets. Agonist activation of GPCRs almost invariably results in the receptor being desensiti...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 33(2002), 5 vom: 21. Nov., Seite 1152-4, 1156-7
1. Verfasser: Adie, E J (VerfasserIn)
Weitere Verfasser: Kalinka, S, Smith, L, Francis, M J, Marenghi, A, Cooper, M E, Briggs, M, Michael, N P, Milligan, G, Game, S
Format: Aufsatz
Sprache:English
Veröffentlicht: 2002
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Evaluation Study Journal Article Antibodies, Anti-Idiotypic Carbocyanines CypHer 5 NHS ester Epitopes Fluorescent Dyes G protein, vesicular stomatitis virus Luminescent Proteins Membrane Glycoproteins mehr... PTGIR protein, human Ptgir protein, mouse Receptors, Epoprostenol Receptors, Opioid, delta Receptors, Prostaglandin Receptors, Thyrotropin-Releasing Hormone Recombinant Fusion Proteins Viral Envelope Proteins anti-IgG Green Fluorescent Proteins 147336-22-9 Enkephalin, Leucine-2-Alanine 63631-40-3 Thyrotropin 9002-71-5 GTP-Binding Proteins EC 3.6.1.- Iloprost JED5K35YGL