Broad-host-range mobilizable suicide vectors for promoter trapping in gram-negative bacteria
Here we report the construction of three different vectors for the identification of bacterial genes induced in vitro and/or in vivo. These plasmids contain kanamycin, gentamicin, or tetracycline resistance genes as selectable markers. A promoterless cat and an improved GFP (mut3-gfp) can be used to...
| Publié dans: | BioTechniques. - 1991. - 33(2002), 5 vom: 21. Nov., Seite 1038-40, 1042-3 |
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| Auteur principal: | |
| Autres auteurs: | , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2002
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article DNA-Binding Proteins Luminescent Proteins Trans-Activators replication initiator protein Green Fluorescent Proteins 147336-22-9 Chloramphenicol O-Acetyltransferase EC 2.3.1.28 DNA Helicases |
| Résumé: | Here we report the construction of three different vectors for the identification of bacterial genes induced in vitro and/or in vivo. These plasmids contain kanamycin, gentamicin, or tetracycline resistance genes as selectable markers. A promoterless cat and an improved GFP (mut3-gfp) can be used to follow the induction of gene expression by measuring chloramphenicol resistance and fluorescence, respectively |
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| Description: | Date Completed 17.06.2003 Date Revised 28.09.2018 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |