Creating random mutagenesis libraries using megaprimer PCR of whole plasmid
The conventional method for cloning a DNA fragment is to insert it into a vector and ligate it. Although this method is commonly used, it is labor intensive because the ratio and concentrations of the DNA insert and the vector need optimizing. Even then, the resultant library is often plagued with u...
| Publié dans: | BioTechniques. - 1993. - 33(2002), 5 vom: 21. Nov., Seite 1033-4, 1036-8 |
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| Format: | Article |
| Langue: | English |
| Publié: |
2002
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| Accès à la collection: | BioTechniques |
| Sujets: | Evaluation Study Journal Article DNA Primers DNA, Recombinant Luminescent Proteins Green Fluorescent Proteins 147336-22-9 Pfu DNA polymerase EC 2.7.7.- Taq Polymerase plus... |