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|a pubmed24n1221.xml
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|a (DE-627)NLM122186710
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|a (NLM)12432037
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Miyazawa, Yutaka
|e verfasserin
|4 aut
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|a Isolation and expression of a novel starch-storing cell-specific gene containing the KH RNA binding domain from tobacco-cultured cells BY-2
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|c 2002
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 30.01.2003
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|a Date Revised 13.12.2023
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|a published: Print
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|a GENBANK: AB088637
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|a Citation Status MEDLINE
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|a In cultured Bright Yellow-2 tobacco (Nicotiana tabacum) cells, the depletion of 2,4-dichlorophenoxyacetic acid (2,4-D) in the culture medium induces amyloplast development. This differentiation also includes a decrease in cell multiplication, and an increase in cell size. These changes were primarily triggered by the depletion of 2,4-D, and accelerated by the addition of benzyladenine (BA). Three cDNAs were identified whose transcript levels are specifically increased during differentiation of starch-storing cells using the differential display method, and designated as starch-storing cell induced genes (SCI genes). One of these cDNAs, SCI2 encodes a 285 amino acids long protein with a KH RNA-binding domain. A database search revealed that this protein showed similarity to respective domains of mammalian quaking proteins. 2,4-D addition, which can convert starch-storing cells into dividing cells, to starch-storing BY-2 cells, immediately decreases the SCI2 transcript level, suggesting that SCI2 may have some role in starch-storing cell differentiation in BY-2 cells
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Culture Media
|2 NLM
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|a DNA, Complementary
|2 NLM
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|a RNA-Binding Proteins
|2 NLM
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|a 2,4-Dichlorophenoxyacetic Acid
|2 NLM
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|a 2577AQ9262
|2 NLM
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|a Starch
|2 NLM
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|a 9005-25-8
|2 NLM
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|a Sakai, Atsushi
|e verfasserin
|4 aut
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|a Matsunaga, Sachihiro
|e verfasserin
|4 aut
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|a Asami, Tadao
|e verfasserin
|4 aut
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|a Kawano, Shigeyuki
|e verfasserin
|4 aut
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|a Kuroiwa, Tsuneyoshi
|e verfasserin
|4 aut
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|a Yoshida, Shigeo
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 53(2002), 379 vom: 21. Dez., Seite 2451-2
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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|g volume:53
|g year:2002
|g number:379
|g day:21
|g month:12
|g pages:2451-2
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|a GBV_USEFLAG_A
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|a GBV_ILN_350
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|a AR
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|d 53
|j 2002
|e 379
|b 21
|c 12
|h 2451-2
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