Isolation and expression of a novel starch-storing cell-specific gene containing the KH RNA binding domain from tobacco-cultured cells BY-2

In cultured Bright Yellow-2 tobacco (Nicotiana tabacum) cells, the depletion of 2,4-dichlorophenoxyacetic acid (2,4-D) in the culture medium induces amyloplast development. This differentiation also includes a decrease in cell multiplication, and an increase in cell size. These changes were primaril...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 53(2002), 379 vom: 21. Dez., Seite 2451-2
1. Verfasser: Miyazawa, Yutaka (VerfasserIn)
Weitere Verfasser: Sakai, Atsushi, Matsunaga, Sachihiro, Asami, Tadao, Kawano, Shigeyuki, Kuroiwa, Tsuneyoshi, Yoshida, Shigeo
Format: Aufsatz
Sprache:English
Veröffentlicht: 2002
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Culture Media DNA, Complementary RNA-Binding Proteins 2,4-Dichlorophenoxyacetic Acid 2577AQ9262 Starch 9005-25-8
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245 1 0 |a Isolation and expression of a novel starch-storing cell-specific gene containing the KH RNA binding domain from tobacco-cultured cells BY-2 
264 1 |c 2002 
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500 |a Date Revised 13.12.2023 
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500 |a Citation Status MEDLINE 
520 |a In cultured Bright Yellow-2 tobacco (Nicotiana tabacum) cells, the depletion of 2,4-dichlorophenoxyacetic acid (2,4-D) in the culture medium induces amyloplast development. This differentiation also includes a decrease in cell multiplication, and an increase in cell size. These changes were primarily triggered by the depletion of 2,4-D, and accelerated by the addition of benzyladenine (BA). Three cDNAs were identified whose transcript levels are specifically increased during differentiation of starch-storing cells using the differential display method, and designated as starch-storing cell induced genes (SCI genes). One of these cDNAs, SCI2 encodes a 285 amino acids long protein with a KH RNA-binding domain. A database search revealed that this protein showed similarity to respective domains of mammalian quaking proteins. 2,4-D addition, which can convert starch-storing cells into dividing cells, to starch-storing BY-2 cells, immediately decreases the SCI2 transcript level, suggesting that SCI2 may have some role in starch-storing cell differentiation in BY-2 cells 
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700 1 |a Sakai, Atsushi  |e verfasserin  |4 aut 
700 1 |a Matsunaga, Sachihiro  |e verfasserin  |4 aut 
700 1 |a Asami, Tadao  |e verfasserin  |4 aut 
700 1 |a Kawano, Shigeyuki  |e verfasserin  |4 aut 
700 1 |a Kuroiwa, Tsuneyoshi  |e verfasserin  |4 aut 
700 1 |a Yoshida, Shigeo  |e verfasserin  |4 aut 
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