Fluorescence-based, high-throughput DNA polymerase assay
The commonly used DNA polymerase assay is based on the detection of incorporated radiolabeled nucleotides in a DNA elongation reaction. It is laborious, radioactive, and can be highly variable. Here we report a nonradioactive fluorescence-based assay. The method consists of Cydye-labeled nucleotides...
| Veröffentlicht in: | BioTechniques. - 1993. - 33(2002), 4 vom: 14. Okt., Seite 938-41 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2002
|
| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Comparative Study Journal Article DNA Primers Taq Polymerase EC 2.7.7.- bacteriophage T7 induced DNA polymerase DNA-Directed DNA Polymerase EC 2.7.7.7 |