Cloning of cDNA encoding COMT from wheat which is differentially expressed in lodging-sensitive and -resistant cultivars

In the present study two cDNA fragments were cloned by nested-PCR using degenerate primers for COMT and found to be 93% identical at the nucleotide level. The deduced amino acid sequence of the two cDNAs showed a high degree of identity with COMT from other plants and were most similar to COMTs from...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 53(2002), 378 vom: 29. Nov., Seite 2281-2
1. Verfasser: Ma, Qing-Hu (VerfasserIn)
Weitere Verfasser: Xu, Yang, Lin, Zhan-Bing, He, Ping
Format: Aufsatz
Sprache:English
Veröffentlicht: 2002
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA, Complementary Lignin 9005-53-2 Methyltransferases EC 2.1.1.- caffeate O-methyltransferase EC 2.1.1.68
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245 1 0 |a Cloning of cDNA encoding COMT from wheat which is differentially expressed in lodging-sensitive and -resistant cultivars 
264 1 |c 2002 
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520 |a In the present study two cDNA fragments were cloned by nested-PCR using degenerate primers for COMT and found to be 93% identical at the nucleotide level. The deduced amino acid sequence of the two cDNAs showed a high degree of identity with COMT from other plants and were most similar to COMTs from monocots. RNA gel blot hybridization demonstrated that the wheat COMT gene W-cm5-1 was expressed in stem, root and leaf tissues. W-cm5-1 mRNA levels in the developing wheat stem were associated with the stem lodging character in two wheat cultivars. These results suggest that the action of the wheat COMT gene may be related to stem rigidity and lodging character in wheat 
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650 4 |a Research Support, Non-U.S. Gov't 
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700 1 |a Lin, Zhan-Bing  |e verfasserin  |4 aut 
700 1 |a He, Ping  |e verfasserin  |4 aut 
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