A trans-zeatin riboside in root xylem sap negatively regulates adventitious root formation on cucumber hypocotyls
Shoot cultures of cucumber were used to analyse the roles of root-derived substances in adventitious root formation on hypocotyl tissues. Xylem sap collected from the roots of squash had a strong inhibitory effect on the formation of hypocotyl adventitious roots. Double-solvent extraction followed b...
| Veröffentlicht in: | Journal of experimental botany. - 1985. - 53(2002), 378 vom: 29. Nov., Seite 2193-200 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2002
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| Zugriff auf das übergeordnete Werk: | Journal of experimental botany |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Cytokinins Isopentenyladenosine 7724-76-7 zeatin riboside 7LG4D082A9 Adenosine K72T3FS567 |
| Zusammenfassung: | Shoot cultures of cucumber were used to analyse the roles of root-derived substances in adventitious root formation on hypocotyl tissues. Xylem sap collected from the roots of squash had a strong inhibitory effect on the formation of hypocotyl adventitious roots. Double-solvent extraction followed by fractionation with both normal and reverse phase column chromatographies and analysis by liquid chromatography/tandem mass spectrometry identified trans-zeatin riboside (ZR) as the primary suppressor of adventitious root formation. ZR was the predominant cytokinin present in the xylem sap, occurring at a concentration of 2x10(-8 )M. Application of ZR at concentrations from 3.16x10(-9) M effected inhibition of adventitious root formation. These results suggest that ZR transported from roots via xylem sap may act as an endogenous suppressor of hypocotyl adventitious root formation in planta |
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| Beschreibung: | Date Completed 21.01.2003 Date Revised 13.05.2019 published: Print Citation Status MEDLINE |
| ISSN: | 1460-2431 |