Affinity tag for protein purification and detection based on the disulfide-linked complex of InaD and NorpA

Affinity tags are not only used for the expression and purification of recombinant proteins but also for the detection of protein-protein interactions. Common problems with many affinity tags are excessive length, which may interfere with the structure and function of tagged proteins, and low affini...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 33(2002), 3 vom: 11. Sept., Seite 578, 580, 584-8 passim
1. Verfasser: Kimple, Michelle E (VerfasserIn)
Weitere Verfasser: Sondek, John
Format: Aufsatz
Sprache:English
Veröffentlicht: 2002
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Affinity Labels Disulfides Drosophila Proteins Eye Proteins Macromolecular Substances Proteins mehr... inaD protein, Drosophila Type C Phospholipases EC 3.1.4.- NorpA protein, Drosophila EC 3.1.4.11 Phospholipase C beta Phosphatidylinositol Diacylglycerol-Lyase EC 4.6.1.13