Thirty-cycle temperature optimization of a closed-cycle capillary PCR machine
The performance of a novel thermal cycler has been characterized in a 30-cycle PCR. The device consists of a microcapillary equipped with bidirectional pressure-driven flow and in situ optical position sensors. A 1-microL droplet of reaction mixture moves between three heat zones in a 1-mm i.d., oil...
| Veröffentlicht in: | BioTechniques. - 1993. - 33(2002), 3 vom: 11. Sept., Seite 557-8, 560, 562 passim |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2002
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Comparative Study Evaluation Study Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article DNA Primers DNA, Bacterial Taq Polymerase EC 2.7.7.- |