Resuspension of DNA sequencing reaction products in agarose increases sequence quality on an automated sequencer

Resuspension of DNA sequencing reaction products in agarose increases sequence quality on an automated sequencer

We are investigating approaches to increase DNA sequencing quality. Since a majorfactor in sequence generation is the cost of reagents and sample preparations, we have developed and optimized methods to sequence directly plasmid DNA isolated from alkaline lysis preparations. These methods remove the...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 33(2002), 3 vom: 11. Sept., Seite 532-4, 536, 538-9
1. Verfasser: Vatcher, G (VerfasserIn)
Weitere Verfasser: Smailus, D, Krzywinski, M, Guin, R, Stott, J, Tsai, M, Chan, S, Pandoh, P, Yang, G, Asano, J, Olson, T, Prabhu, A L, Coope, R, Marziali, A, Schein, J, Jones, S, Marra, M
Format: Aufsatz
Sprache:English
Veröffentlicht: 2002
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Evaluation Study Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Technical Report DNA, Bacterial Sepharose 9012-36-6