Isolation and characterization of the ethynylestradiol-biodegrading microorganism Fusarium proliferatum strain HNS-1

We cultivated hundreds of sediment, soil, and manure samples taken from rivers and farms in a medium containing ethynylestradiol (EE2) as the sole source of carbon, so that microorganisms in the samples would acclimatize to the presence of EE2. Finally, we isolated an EE2-degrading microorganism, de...

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Détails bibliographiques
Publié dans:Water science and technology : a journal of the International Association on Water Pollution Research. - 1986. - 45(2002), 12 vom: 07., Seite 175-9
Auteur principal: Shi, J H (Auteur)
Autres auteurs: Suzuki, Y, Lee, B D, Nakai, S, Hosomi, M
Format: Article
Langue:English
Publié: 2002
Accès à la collection:Water science and technology : a journal of the International Association on Water Pollution Research
Sujets:Journal Article Research Support, Non-U.S. Gov't Estradiol Congeners Manure Water Pollutants, Chemical Ethinyl Estradiol 423D2T571U
Description
Résumé:We cultivated hundreds of sediment, soil, and manure samples taken from rivers and farms in a medium containing ethynylestradiol (EE2) as the sole source of carbon, so that microorganisms in the samples would acclimatize to the presence of EE2. Finally, we isolated an EE2-degrading microorganism, designated as strain HNS-1, from a cowshed sample. Based on its partial nucleotide sequence (563 bp) of the 28S rRNA gene, strain HNS-1 was identified as Fusarium proliferatum. Over 15 days, F. proliferatum strain HNS-1 removed 97% of EE2 at an initial concentration of 25 mg.L-1, with a first-order rate constant of 0.6 d-1. Unknown products of EE2 degradation, which may be more polar compounds that have a phenolic group, remained in the culture medium
Description:Date Completed 15.01.2003
Date Revised 21.11.2013
published: Print
Citation Status MEDLINE
ISSN:0273-1223