Isolation and characterization of the ethynylestradiol-biodegrading microorganism Fusarium proliferatum strain HNS-1
We cultivated hundreds of sediment, soil, and manure samples taken from rivers and farms in a medium containing ethynylestradiol (EE2) as the sole source of carbon, so that microorganisms in the samples would acclimatize to the presence of EE2. Finally, we isolated an EE2-degrading microorganism, de...
Publié dans: | Water science and technology : a journal of the International Association on Water Pollution Research. - 1986. - 45(2002), 12 vom: 07., Seite 175-9 |
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Auteur principal: | |
Autres auteurs: | , , , |
Format: | Article |
Langue: | English |
Publié: |
2002
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Accès à la collection: | Water science and technology : a journal of the International Association on Water Pollution Research |
Sujets: | Journal Article Research Support, Non-U.S. Gov't Estradiol Congeners Manure Water Pollutants, Chemical Ethinyl Estradiol 423D2T571U |
Résumé: | We cultivated hundreds of sediment, soil, and manure samples taken from rivers and farms in a medium containing ethynylestradiol (EE2) as the sole source of carbon, so that microorganisms in the samples would acclimatize to the presence of EE2. Finally, we isolated an EE2-degrading microorganism, designated as strain HNS-1, from a cowshed sample. Based on its partial nucleotide sequence (563 bp) of the 28S rRNA gene, strain HNS-1 was identified as Fusarium proliferatum. Over 15 days, F. proliferatum strain HNS-1 removed 97% of EE2 at an initial concentration of 25 mg.L-1, with a first-order rate constant of 0.6 d-1. Unknown products of EE2 degradation, which may be more polar compounds that have a phenolic group, remained in the culture medium |
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Description: | Date Completed 15.01.2003 Date Revised 21.11.2013 published: Print Citation Status MEDLINE |
ISSN: | 0273-1223 |