|
|
|
|
| LEADER |
01000caa a22002652c 4500 |
| 001 |
NLM120261154 |
| 003 |
DE-627 |
| 005 |
20250203100344.0 |
| 007 |
tu |
| 008 |
231222s2002 xx ||||| 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed25n0401.xml
|
| 035 |
|
|
|a (DE-627)NLM120261154
|
| 035 |
|
|
|a (NLM)12165276
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Chang, Wei-Kuo
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Glutamine protects activated human T cells from apoptosis by up-regulating glutathione and Bcl-2 levels
|
| 264 |
|
1 |
|c 2002
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
|
| 338 |
|
|
|a Band
|b nc
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 10.10.2002
|
| 500 |
|
|
|a Date Revised 06.11.2019
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Glutamine is the most abundant amino acid in the body. A decrease of plasma glutamine concentrations is found in catabolic stress and is related to susceptibility to infections. Glutamine is known to modulate lymphocyte activation; however, little is known about glutamine modulation of cell death of activated human T cells. Using Jurkat T cells, we investigated glutamine modulation of T-cell apoptosis activated by PMA plus ionomycin. We found that glutamine at various concentrations significantly enhanced IL-2 production, cell proliferation, and cell viability of Jurkat T cells. Glutamine also decreased the number of apoptotic cells stimulated with PMA plus ionomycin as demonstrated by flow cytometry. Meanwhile, glutamine down-regulated CD95 and CD95L expression, but up-regulated CD45RO and Bcl-2 expression in activated T cells. Further investigation of CD95-mediated caspase activities revealed that supplementation of glutamine significantly decreased caspase-3 and caspase-8 activities in activated T cells. Since oxidative stress is closely associated with induction of lymphocyte apoptosis, we found that glutamine significantly increased glutathione (GSH), but decreased reactive oxygen species levels in activated T cells. Blockade of intracellular GSH formation enhanced, but exogenous GSH supplementation decreased, activated T-cell apoptosis. Studying normal peripheral lymphoproliferation, we also found that the presence of glutamine increased lymphoproliferation as well as Bcl-2 and CD95 expression; but decreased CD95L and activation-induced T-cell death. Taken together, glutamine appeared to augment lymphoproliferation but suppressed activation-induced T-cell death in both Jurkat T cells and human peripheral T lymphocytes
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
7 |
|a FASLG protein, human
|2 NLM
|
| 650 |
|
7 |
|a Fas Ligand Protein
|2 NLM
|
| 650 |
|
7 |
|a Interleukin-2
|2 NLM
|
| 650 |
|
7 |
|a Membrane Glycoproteins
|2 NLM
|
| 650 |
|
7 |
|a Proto-Oncogene Proteins c-bcl-2
|2 NLM
|
| 650 |
|
7 |
|a fas Receptor
|2 NLM
|
| 650 |
|
7 |
|a Glutamine
|2 NLM
|
| 650 |
|
7 |
|a 0RH81L854J
|2 NLM
|
| 650 |
|
7 |
|a Ionomycin
|2 NLM
|
| 650 |
|
7 |
|a 56092-81-0
|2 NLM
|
| 650 |
|
7 |
|a Leukocyte Common Antigens
|2 NLM
|
| 650 |
|
7 |
|a EC 3.1.3.48
|2 NLM
|
| 650 |
|
7 |
|a Glutathione
|2 NLM
|
| 650 |
|
7 |
|a GAN16C9B8O
|2 NLM
|
| 650 |
|
7 |
|a Tetradecanoylphorbol Acetate
|2 NLM
|
| 650 |
|
7 |
|a NI40JAQ945
|2 NLM
|
| 700 |
1 |
|
|a Yang, Kuender D
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Chuang, Hau
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Jan, Jia-Tsong
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Shaio, Men-Fang
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 104(2002), 2 vom: 06. Aug., Seite 151-60
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnas
|
| 773 |
1 |
8 |
|g volume:104
|g year:2002
|g number:2
|g day:06
|g month:08
|g pages:151-60
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_11
|
| 912 |
|
|
|a GBV_ILN_24
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 104
|j 2002
|e 2
|b 06
|c 08
|h 151-60
|