RT-PCR heteroduplex analysis permits differentiation of transgene and host gene expression in a transgenic animal model

RT-PCR heteroduplex analysis permits differentiation of transgene and host gene expression in a transgenic animal model

In transgenic animal models, the conservation of DNA sequences between the transgene and the host wild-type gene can complicate the evaluation of the expression of each gene. The potential for gene silencing may complicate matters further. Here we report the use of RT-PCR heteroduplex analysis to di...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 33(2002), 1 vom: 12. Juli, Seite 58, 60-2, 64 passim
1. Verfasser: Duan, W (VerfasserIn)
Weitere Verfasser: Ding, H, Zhu, W G, Srinivasan, K, Otterson, G A, Villalona-Calero, M A, Ottersom, G A
Format: Aufsatz
Sprache:English
Veröffentlicht: 2002
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article