Truncated amplification : a method for high-fidelity template-driven nucleic acid amplification
The error rate of conventional PCR is problematic when amplifying from single cells or amplifying segments for protein functional analysis by in vitro translation. We describe truncated amplification, a method for high-fidelity amplification in which DNA polymerase errors are not propagated efficien...
| Veröffentlicht in: | BioTechniques. - 1993. - 33(2002), 1 vom: 12. Juli, Seite 129-32, 134-6, 138 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2002
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article DNA Primers Pfu DNA polymerase EC 2.7.7.- Taq Polymerase Tli polymerase DNA-Directed DNA Polymerase EC 2.7.7.7 |