Cloning of a tobacco cDNA coding for a putative transcriptional coactivator MBF1 that interacts with the tomato mosaic virus movement protein

Viral movement through plasmodesmata in host plants depends on the interaction between virus-encoded movement protein (MP) and host proteins. To search for MP-interacting protein (MIP), far-western screening of a tobacco cDNA library was carried out using a recombinant MP of tomato mosaic virus (ToM...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 53(2002), 373 vom: 30. Juni, Seite 1531-2
1. Verfasser: Matsushita, Yasuhiko (VerfasserIn)
Weitere Verfasser: Miyakawa, Orie, Deguchi, Masakazu, Nishiguchi, Masamichi, Nyunoya, Hiroshi
Format: Aufsatz
Sprache:English
Veröffentlicht: 2002
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Arabidopsis Proteins DNA, Complementary MBF1 protein, Arabidopsis Plant Viral Movement Proteins Trans-Activators Viral Proteins
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100 1 |a Matsushita, Yasuhiko  |e verfasserin  |4 aut 
245 1 0 |a Cloning of a tobacco cDNA coding for a putative transcriptional coactivator MBF1 that interacts with the tomato mosaic virus movement protein 
264 1 |c 2002 
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500 |a Date Revised 13.12.2023 
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520 |a Viral movement through plasmodesmata in host plants depends on the interaction between virus-encoded movement protein (MP) and host proteins. To search for MP-interacting protein (MIP), far-western screening of a tobacco cDNA library was carried out using a recombinant MP of tomato mosaic virus (ToMV) as a probe. One of the positive cDNA clones, designated MIP204, was highly homologous to a class of transcriptional coactivators commonly referred to as multiprotein bridging factor 1 (MBF1). ToMV MP could also bind to the Arabidopsis homologues of MBF1. The recombinant MIP204 bound to MPs of ToMV and a crucifer tobamovirus CTMV-W, but not of cucumber mosaic virus. MPs of ToMV and the related virus may interact with MBF1-like proteins to modulate host gene expression 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a DNA, Complementary  |2 NLM 
650 7 |a MBF1 protein, Arabidopsis  |2 NLM 
650 7 |a Plant Viral Movement Proteins  |2 NLM 
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650 7 |a Viral Proteins  |2 NLM 
700 1 |a Miyakawa, Orie  |e verfasserin  |4 aut 
700 1 |a Deguchi, Masakazu  |e verfasserin  |4 aut 
700 1 |a Nishiguchi, Masamichi  |e verfasserin  |4 aut 
700 1 |a Nyunoya, Hiroshi  |e verfasserin  |4 aut 
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