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20231222183113.0 |
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231222s2002 xx ||||| 00| ||eng c |
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|a pubmed24n0396.xml
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|a (NLM)11987982
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Padilla, Josué
|e verfasserin
|4 aut
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| 245 |
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|a Human B lymphocytes and B lymphomas express PPAR-gamma and are killed by PPAR-gamma agonists
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| 264 |
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|c 2002
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 23.05.2002
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|a Date Revised 21.11.2013
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|a published: Print
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|a Citation Status MEDLINE
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|a This paper evaluates the expression and functional significance of PPAR-gamma on human B cells. Recent interest in PPAR-gamma has focused on its adipogenic effects on non-bone marrow-derived cells. PPAR-gamma agonists also have been proposed as anti-inflammatory agents owing to inhibition of NF-kappa B activation. We report herein the first study evaluating PPAR-gamma expression and functional significance in human B lineage cells. Interestingly, normal human B cells and a variety of B lymphoma cells (e.g., Daudi, Ramos, and Raji) express PPAR-gamma protein as determined by immunocytochemistry. The expression of 80-kDa PPAR-gamma on human B lymphocytes and B lymphomas was confirmed by Western blot analysis. 15-Deoxy-Delta(12,14)-PGJ(2) (15d-PGJ(2)), a natural PPAR-gamma agonist, has a dose-dependent antiproliferative and cytotoxic effect on normal and malignant B cells as shown by [(3)H]thymidine and MTT assays. Only PPAR-gamma agonists (thiazolidinediones) and not PPAR-alpha agonists mimicked the effect of 15d-PGJ(2) on B lineage cells, indicating that the mechanism by which 15d-PGJ(2) negatively affects B lineage cells involves, in part, PPAR-gamma. The mechanism whereby PPAR-gamma agonists induce cytotoxicity is via apoptosis as shown by Annexin V staining and as confirmed by DNA fragmentation detected using the TUNEL assay. This is the first study evaluating PPAR-gamma expression and its significance on human B lymphocytes. PPAR-gamma agonists may serve as a counterbalance to the stimulating effects of other prostaglandins, namely PGE(2), which promotes B cell immunoglobulin class switching. Finally, the use of prostaglandins such as 15d-PGJ(2) and synthetic PPAR-gamma agonists to induce apoptosis in B lineage cells may lead to the development of novel therapies for potentially fatal B lymphomas
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Research Support, U.S. Gov't, P.H.S.
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| 650 |
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|a 15-deoxy-delta(12,14)-prostaglandin J2
|2 NLM
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| 650 |
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|a Receptors, Cytoplasmic and Nuclear
|2 NLM
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| 650 |
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|a Transcription Factors
|2 NLM
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|a Prostaglandin D2
|2 NLM
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| 650 |
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|a RXY07S6CZ2
|2 NLM
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| 700 |
1 |
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|a Leung, Edmund
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Phipps, Richard P
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 103(2002), 1 vom: 30. Apr., Seite 22-33
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
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| 773 |
1 |
8 |
|g volume:103
|g year:2002
|g number:1
|g day:30
|g month:04
|g pages:22-33
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