Low efficiency of the Moloney murine leukemia virus reverse transcriptase during reverse transcription of rare t(8;21) fusion gene transcripts

Low efficiency of the Moloney murine leukemia virus reverse transcriptase during reverse transcription of rare t(8;21) fusion gene transcripts

The resolving power of RT-PCR is limited by the efficiency of RNA-to-cDNA conversion. Methods to determine this efficiency, using a real-time PCR assay for quantifying AML1-MTG 8 [t(8;21)] fusion gene transcripts, are described. The efficiency is shown to be directly proportional to RNA template lev...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 32(2002), 4 vom: 05. Apr., Seite 768, 770, 772, 754-5
1. Verfasser: Curry, John (VerfasserIn)
Weitere Verfasser: McHale, Cliona, Smith, Martyn T
Format: Aufsatz
Sprache:English
Veröffentlicht: 2002
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article AML1-ETO fusion protein, human Core Binding Factor Alpha 2 Subunit Oncogene Proteins, Fusion RNA, Messenger RUNX1 Translocation Partner 1 Protein Transcription Factors mehr... RNA-Directed DNA Polymerase EC 2.7.7.49