IDI7, a new iron-regulated ABC transporter from barley roots, localizes to the tonoplast

A new Fe-deficiency-induced cDNA, IDI7, was isolated from the roots of Fe-deficient barley (Hordeum vulgare L. cv. Ehimehadaka no. 1). The transcript levels of IDI7 in roots strongly correlated with iron nutritional status, and induction by Fe-deficiency was restricted to roots. Excess treatment wit...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 53(2002), 369 vom: 15. Apr., Seite 727-35
1. Verfasser: Yamaguchi, Hirotaka (VerfasserIn)
Weitere Verfasser: Nishizawa, Naoko-Kishi, Nakanishi, Hiromi, Mori, Satoshi
Format: Aufsatz
Sprache:English
Veröffentlicht: 2002
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article ATP-Binding Cassette Transporters DNA, Complementary Luminescent Proteins Plant Proteins Recombinant Fusion Proteins Green Fluorescent Proteins 147336-22-9 Manganese 42Z2K6ZL8P mehr... Copper 789U1901C5 Iron E1UOL152H7 Zinc J41CSQ7QDS
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100 1 |a Yamaguchi, Hirotaka  |e verfasserin  |4 aut 
245 1 0 |a IDI7, a new iron-regulated ABC transporter from barley roots, localizes to the tonoplast 
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500 |a Date Completed 23.05.2002 
500 |a Date Revised 13.12.2023 
500 |a published: Print 
500 |a Citation Status MEDLINE 
520 |a A new Fe-deficiency-induced cDNA, IDI7, was isolated from the roots of Fe-deficient barley (Hordeum vulgare L. cv. Ehimehadaka no. 1). The transcript levels of IDI7 in roots strongly correlated with iron nutritional status, and induction by Fe-deficiency was restricted to roots. Excess treatment with heavy metal ions, such as copper, manganese, and zinc, did not cause obvious IDI7 induction in either leaves or roots. IDI7 encodes a 644 amino acid protein, and has features typical of ATP-binding cassette (ABC) transporters. Phylogenetic analysis revealed that IDI7 is closely related to the half-type ABC protein subfamily, which includes mammalian transporters associated with antigen processing (TAPs). A transiently expressed fusion protein of IDI7 to green fluorescent protein (GFP) was localized to tonoplasts in suspension-cultured tobacco (Nicotiana tabacum L.) cells. IDI7 and its orthologues are thought to comprise a new class of ABC transporters, located in the tonoplasts of higher plants. A possible Fe-deficiency adaptation role for IDI7 in barley root cells, involving transport across the tonoplast, is proposed 
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650 7 |a DNA, Complementary  |2 NLM 
650 7 |a Luminescent Proteins  |2 NLM 
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650 7 |a Recombinant Fusion Proteins  |2 NLM 
650 7 |a Green Fluorescent Proteins  |2 NLM 
650 7 |a 147336-22-9  |2 NLM 
650 7 |a Manganese  |2 NLM 
650 7 |a 42Z2K6ZL8P  |2 NLM 
650 7 |a Copper  |2 NLM 
650 7 |a 789U1901C5  |2 NLM 
650 7 |a Iron  |2 NLM 
650 7 |a E1UOL152H7  |2 NLM 
650 7 |a Zinc  |2 NLM 
650 7 |a J41CSQ7QDS  |2 NLM 
700 1 |a Nishizawa, Naoko-Kishi  |e verfasserin  |4 aut 
700 1 |a Nakanishi, Hiromi  |e verfasserin  |4 aut 
700 1 |a Mori, Satoshi  |e verfasserin  |4 aut 
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