Solid-phase PCR in microwells effects of linker length and composition on tethering, hybridization, and extension

Solid-phase PCR in microwells : effects of linker length and composition on tethering, hybridization, and extension

During the solid-phase PCR (SP-PCR), DNA oligonucleotides complementary to a soluble template and immobilized on a surface are extended in situ. Although primarily used for pathogen detection, SP-PCR has the potential for much broader application, including disease diagnostics, genotyping, and expre...

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Veröffentlicht in:BioTechniques. - 1993. - 32(2002), 2 vom: 25. Feb., Seite 410, 412, 414-8, 420
1. Verfasser: Carmon, A (VerfasserIn)
Weitere Verfasser: Vision, T J, Mitchell, S E, Thannhauser, T W, Müller, U, Kresovich, S
Format: Aufsatz
Sprache:English
Veröffentlicht: 2002
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Evaluation Study Journal Article Research Support, Non-U.S. Gov't Arabidopsis Proteins DNA, Plant Ethylene Glycols phytochrome C, Arabidopsis Phytochrome 11121-56-5 hexaethylene glycol 2615-15-8