Fixation and decalcification of adult zebrafish for histological, immunocytochemical, and genotypic analysis

Fixation and decalcification of adult zebrafish for histological, immunocytochemical, and genotypic analysis

To facilitate the molecular analysis of tissues in adult zebrafish, we tested eight different fixation and decalcification conditions for the ability to yield DNA suitable for PCR and tissue immunoreactivity, following paraffin embedding and sectioning. Although all conditions resulted in good tissu...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 32(2002), 2 vom: 25. Feb., Seite 296-8
1. Verfasser: Moore, Jessica L (VerfasserIn)
Weitere Verfasser: Aros, Michele, Steudel, Kimberly G, Cheng, Keith C
Format: Aufsatz
Sprache:English
Veröffentlicht: 2002
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Evaluation Study Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article Fixatives DNA 9007-49-2