Chimerism analysis in sex-mismatched murine transplantation using quantitative real-time PCR

Chimerism analysis in sex-mismatched murine transplantation using quantitative real-time PCR

Marine experimental stem cell transplantations require the accurate discrimination and quantification of donor cells from host cells. A Y-chromosome-specific, quantitative real-time PCR (kinetic PCR) protocol for blood-derived DNA was developed. The assay sensitivity was extremely high with accurate...

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Publié dans:BioTechniques. - 1991. - 32(2002), 2 vom: 25. Feb., Seite 279-80, 282-4, 286
Auteur principal: Byrne, P (Auteur)
Autres auteurs: Huang, W, Wallace, V M, Shean, M K, Zhang, Z, Zhong, Q, Theodossiou, C, Blakesley, H, Kolls, J K, Schwarzenberger, P
Format: Article
Langue:English
Publié: 2002
Accès à la collection:BioTechniques
Sujets:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. DNA 9007-49-2