Molecular identification using flow cytometry histograms and information theory

Molecular identification using flow cytometry histograms and information theory

Flow cytometry is a common technique for quantitatively measuring the expression of individual molecules on cells. The molecular expression is represented by a frequency histogram of fluorescence intensity. For flow cytometry to be used as a knowledge discovery tool to identify unknown molecules, hi...

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Détails bibliographiques
Publié dans:Proceedings. AMIA Symposium. - 1998. - (2001) vom: 11., Seite 776-80
Auteur principal: Zeng, Q (Auteur)
Autres auteurs: Young, A J, Boxwala, A, Rawn, J, Long, W, Wand, M, Salganik, M, Milford, E L, Mentzer, S J, Greenes, R A
Format: Article
Langue:English
Publié: 2001
Accès à la collection:Proceedings. AMIA Symposium
Sujets:Journal Article
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520 |a Flow cytometry is a common technique for quantitatively measuring the expression of individual molecules on cells. The molecular expression is represented by a frequency histogram of fluorescence intensity. For flow cytometry to be used as a knowledge discovery tool to identify unknown molecules, histogram comparison is a major limitation. Many traditional comparison methods do not provide adequate assessment of histogram similarity and molecular relatedness. We have explored a new approach applying information theory to histogram comparison, and tested it with histograms from 14 antibodies over 3 cell types. The information theory approach was able to improve over traditional methods by recognizing various non-random correlations between histograms in addition to similarity and providing a quantitative assessment of similarity beyond hypothesis testing of identity 
650 4 |a Journal Article 
700 1 |a Young, A J  |e verfasserin  |4 aut 
700 1 |a Boxwala, A  |e verfasserin  |4 aut 
700 1 |a Rawn, J  |e verfasserin  |4 aut 
700 1 |a Long, W  |e verfasserin  |4 aut 
700 1 |a Wand, M  |e verfasserin  |4 aut 
700 1 |a Salganik, M  |e verfasserin  |4 aut 
700 1 |a Milford, E L  |e verfasserin  |4 aut 
700 1 |a Mentzer, S J  |e verfasserin  |4 aut 
700 1 |a Greenes, R A  |e verfasserin  |4 aut 
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