Consensus PCR and microarray for diagnosis of the genus Staphylococcus, species, and methicillin resistance

We propose the use of DNA microarray for the discrimination of homologous products after a single PCR amplification with consensus primers. The method was applied to Staphylococcus identification. The femA nucleotide sequences, which are phylogenetically conserved among the staphylococci, were first...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 31(2001), 6 vom: 29. Dez., Seite 1364-6, 1368, 1370-2
1. Verfasser: Hamels, S (VerfasserIn)
Weitere Verfasser: Gala, J L, Dufour, S, Vannuffel, P, Zammatteo, N, Remacle, J
Format: Aufsatz
Sprache:English
Veröffentlicht: 2001
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Bacterial Proteins Carrier Proteins DNA, Bacterial FemA protein, Bacteria Penicillin-Binding Proteins Peptidyl Transferases EC 2.3.2.12 Hexosyltransferases mehr... EC 2.4.1.- Muramoylpentapeptide Carboxypeptidase EC 3.4.17.8