Reverse transcription slippage over the mRNA secondary structure of the LIP1 gene
The secondary structures in mRNA often cause early termination during the synthesis of cDNA. In an attempt to determine the 5'-untranslated region (UTR) of the gene LIP1 using the RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE), we found that reverse transcriptases skipped over...
| Veröffentlicht in: | BioTechniques. - 1993. - 31(2001), 6 vom: 29. Dez., Seite 1286, 1288, 1290, passim |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2001
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article 5' Untranslated Regions DNA, Complementary Nerve Growth Factors Peptide Fragments RNA, Messenger |