Removal of polyA tails from full-length cDNA libraries for high-efficiency sequencing

Removal of polyA tails from full-length cDNA libraries for high-efficiency sequencing

We have developed a method to overcome sequencing problems caused by the presence of homopolymer stretches, such as polyA/T, in cDNA libraries. PolyA tails are shortened by cleaving before cDNA cloning with type IIS restriction enzymes, such as GsuI, placed next to the oligo-dT used to prime the pol...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 31(2001), 5 vom: 16. Nov., Seite 1042, 1044, 1048-9
1. Verfasser: Shibata, Y (VerfasserIn)
Weitere Verfasser: Carninci, P, Sato, K, Hayatsu, N, Shiraki, T, Ishii, Y, Arakawa, T, Hara, A, Ohsato, N, Izawa, M, Aizawa, K, Itoh, M, Shibata, K, Shinagawa, A, Kawai, J, Ota, Y, Kikuchi, S, Kishimoto, N, Muramatsu, M, Hayashizaki, Y
Format: Aufsatz
Sprache:English
Veröffentlicht: 2001
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA, Complementary Poly A 24937-83-5