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NLM116177829 |
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DE-627 |
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20231222173754.0 |
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231222s2001 xx ||||| 00| ||eng c |
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|a pubmed24n0388.xml
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|a (DE-627)NLM116177829
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|a (NLM)11726221
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Taniguchi, M
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Cellular immune response against tropomyosin isoform 5 in ulcerative colitis
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| 264 |
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|c 2001
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 10.01.2002
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|a Date Revised 21.11.2008
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a (c)2001 Elsevier Science.
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|a We have reported an autoantibody response in ulcerative colitis (UC) against human tropomyosin isoform 5 (hTM5), the predominant colonic epithelial cell hTM isoform. In this report, we determined the number of IFN-gamma-secreting cells (spot-forming cells, SFC) against hTM5 by an enzyme-linked immunospot (ELISPOT) assay. Another cytoskeletal protein, caldesmon, CaD40, was used as a control antigen. Peripheral blood mononuclear cells were separated by a Ficoll density gradient from 28 patients with UC, 13 patients with Crohn's disease (CD), and 9 healthy subjects (HS). The mean (+/-SEM) SFC values against hTM5 in UC, CD, and HS were 48.8 +/- 8.1, 18.6 +/- 4.6, and 20.8 +/- 8.6, respectively. The value in UC was significantly higher than those in CD (P < 0.005) and HS (P < 0.025). SFC values in CD did not differ from those in HS. None of the 50 samples (except 1 UC) reacted to the CaD40 antigen. This study demonstrates, for the first time, a defined colon epithelial cell antigen, hTM5, that is capable of inducing a significant T cell response in UC but not in CD
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| 650 |
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|a Journal Article
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| 650 |
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4 |
|a Research Support, U.S. Gov't, P.H.S.
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| 650 |
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7 |
|a Autoantibodies
|2 NLM
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| 650 |
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7 |
|a Calmodulin-Binding Proteins
|2 NLM
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| 650 |
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7 |
|a TPM3 protein, human
|2 NLM
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| 650 |
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7 |
|a Tropomyosin
|2 NLM
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| 650 |
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7 |
|a Interferon-gamma
|2 NLM
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| 650 |
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7 |
|a 82115-62-6
|2 NLM
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| 700 |
1 |
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|a Geng, X
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Glazier, K D
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Dasgupta, A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lin, J J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Das, K M
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 101(2001), 3 vom: 07. Dez., Seite 289-95
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
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| 773 |
1 |
8 |
|g volume:101
|g year:2001
|g number:3
|g day:07
|g month:12
|g pages:289-95
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|a AR
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| 952 |
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|d 101
|j 2001
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|h 289-95
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