Picogram cloning and direct in situ sequencing of DNA from gel pieces
We describe a simple and rapid procedure for cloning and sequencing of DNA fragments separated by gel electrophoresis, using novel hydrophilic gels, Clearose BG, Spreadex, and Poly(NAT), that do not melt at 95 degrees C. For cloning, a band of interest is excised precisely and incubated in an extrac...
| Veröffentlicht in: | BioTechniques. - 1993. - 31(2001), 4 vom: 01. Okt., Seite 802-4, 806, 808, 810 |
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| Weitere Verfasser: | , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2001
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't DNA Primers DNA 9007-49-2 Fucosyltransferases EC 2.4.1.- |