Monitoring nuclear import with GFP-variant fusion proteins in digitonin-permeabilized cells
A convenient assay for monitoring nuclear localization signal-mediated nuclear import of green fluorescent protein (GFP)-variant fusion proteins has been developed. This modified assay relies upon indirect immunofluorescence microscopy for visualization of transported substrates. The use of GFP-vari...
| Publié dans: | BioTechniques. - 1991. - 31(2001), 4 vom: 01. Okt., Seite 772-5 |
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| Auteur principal: | |
| Autres auteurs: | |
| Format: | Article |
| Langue: | English |
| Publié: |
2001
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, U.S. Gov't, P.H.S. Luminescent Proteins Recombinant Fusion Proteins Green Fluorescent Proteins 147336-22-9 Digitonin KOO5CM684H |
| Résumé: | A convenient assay for monitoring nuclear localization signal-mediated nuclear import of green fluorescent protein (GFP)-variant fusion proteins has been developed. This modified assay relies upon indirect immunofluorescence microscopy for visualization of transported substrates. The use of GFP-variant fusion proteins allows for the rapid assessment of optimal digitonin concentration and permits nuclear import to be monitored with minimal sample preparation in real time |
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| Description: | Date Completed 08.04.2002 Date Revised 28.09.2018 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |