Determination of optimal non-denaturing elution conditions from affinity columns by a solid-phase screen
The purification of biological macromolecules by affinity chromatography is a widespread technique used to separate a protein from other biological components. However, this method may destroy the protein's physiological activity because elution conditions aimed to dissociate the protein of int...
| Publié dans: | BioTechniques. - 1991. - 31(2001), 3 vom: 14. Sept., Seite 584, 586, 588-90, passim |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
2001
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Apoproteins Buffers Ferric Compounds Indicators and Reagents Receptors, Transferrin Thiocyanates Transferrin apotransferrin plus... |