High-resolution semi-quantitative real-time PCR without the use of a standard curve
The repeatability and sensitivity of a simple, adaptable, semi-quantitative, real-time RT-PCR assay was investigated. The assay can be easily and rapidly applied to quantitate relative levels of any gene product without using standards, provided that amplification conditions are specific for the PCR...
| Publié dans: | BioTechniques. - 1991. - 31(2001), 3 vom: 14. Sept., Seite 502, 504-6, 508 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
2001
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| Accès à la collection: | BioTechniques |
| Sujets: | Research Support, Non-U.S. Gov't Technical Report Journal Article DNA, Complementary RNA, Messenger Tissue Inhibitor of Metalloproteinase-1 |