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231222s1992 xx ||||| 00| ||eng c |
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|a pubmed25n0382.xml
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|a (NLM)11538173
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Raghavan, V
|e verfasserin
|4 aut
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|a Gene activity during germination of spores of the fern, Onoclea sensibilis. Cell-free translation analysis of mRNA of spores and the effect of alpha-amanitin on spore germination
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|c 1992
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 12.10.1995
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| 500 |
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|a Date Revised 30.09.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a Poly(A)-RNA fractions of dormant, dark-imbibed (non-germinating) and photoinduced (germinating) spores of Onoclea sensibilis were poor templates in the rabbit reticulocyte lysate protein synthesizing system, but the translational efficiency of poly(A)+RNA was considerably higher than that of unfractionated RNA. Poly(A)+RNA isolated from photoinduced spores had a consistently higher translational efficiency than poly(A)+RNA from dark-imbibed spores. Analysis of the translation products by one-dimensional polyacrylamide gel electrophoresis showed no qualitative differences in the mRNA populations of dormant, dark-imbibed, and photoinduced spores. However, poly(A)+RNA from dark-imbibed spores appeared to encode in vitro fewer detectable polypeptides at a reduced intensity than photoinduced spores. A DNA clone encoding the large subunit of maize ribulose bisphosphate carboxylase hybridized at strong to moderate intensity to RNA isolated from dark-imbibed spores, indicating the absence of mRNA degradation. Although alpha-amanitin did not inhibit the germination of spores, the drug prevented the elongation of the rhizoid and protonemal initial with a concomitant effect on the synthesis of poly(A)+RNA. These results are consistent with the view that some form of translational control involving stored mRNA operates during dark-imbibition and photoinduced germination of spores
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| 650 |
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|a Journal Article
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| 650 |
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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|a Research Support, U.S. Gov't, P.H.S.
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| 650 |
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|a NASA Discipline Number 29-20
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| 650 |
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|a NASA Discipline Plant Biology
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| 650 |
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|a NASA Program Space Biology
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| 650 |
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|a Non-NASA Center
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| 650 |
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|a Amanitins
|2 NLM
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| 650 |
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|a RNA, Messenger
|2 NLM
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| 650 |
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|a beta-amanitin
|2 NLM
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| 650 |
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|a 21150-22-1
|2 NLM
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| 700 |
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|a Raghavan, V
|e investigator
|4 oth
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 140(1992), 4 vom: 01., Seite 434-40
|w (DE-627)NLM098174622
|x 0176-1617
|7 nnns
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| 773 |
1 |
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|g volume:140
|g year:1992
|g number:4
|g day:01
|g pages:434-40
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