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231222s1992 xx ||||| 00| ||eng c |
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|a pubmed24n1221.xml
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|a (DE-627)NLM114357544
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|a (NLM)11538008
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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100 |
1 |
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|a Henry, R L
|e verfasserin
|4 aut
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1 |
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|a Characterization of photosystem 1 chlorophyll a/b-binding apoprotein accumulation in developing soybean using type-specific antibodies
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|c 1992
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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500 |
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|a Date Completed 14.06.1995
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|a Date Revised 13.12.2023
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|a published: Print
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|a Citation Status MEDLINE
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|a The structure and supramolecular assembly of the soybean photosystem 1 (PS 1) chlorophyll a/b-binding antenna (LHC 1) was examined. We identified the subunit composition of LHC 1 in soybean and followed the accumulation of individual subunits during light-induced assembly. We observed four LHC 1 subunits, at 23, 22, 21 and 20.5 kDa, obtained partial sequence information by amino-terminal sequence analysis, and classified the 20.5, 22, and 21 kDa subunits as being encoded by type I, II, and IV chlorophyll a/b binding protein genes, respectively. Antisera against LHC 1 subunits were used to follow the accumulation of individual subunits during the light-initiated transition from etioplast to chloroplast. Several points are noteworthy. First, monospecific antibody against the 22 kDa subunit decorated a 25 kDa peptide in etiolated tissue, which declined during maturation. This decline correlated with the light-induced appearance of mature 22 kDa peptide, suggesting a precursor/product relationship. Second, the same antibody identified a 22 kDa protein in mature corn, but not a larger band in etiolated corn, suggesting that LHC 1 accumulation is regulated differently between species before the onset of chlorophyll biosynthesis. Third, the mature 22 kDa subunit appeared somewhat later than the other LHC 1 peptides during greening, implying that this subunit is less intimately associated with the PS1 core than are the subunits appearing earlier in development
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650 |
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|a Comparative Study
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4 |
|a Journal Article
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4 |
|a Research Support, Non-U.S. Gov't
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650 |
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4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
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650 |
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|a Research Support, U.S. Gov't, P.H.S.
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650 |
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|a NASA Discipline Cell Biology
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650 |
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|a NASA Discipline Developmental Biology
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|a NASA Discipline Number 93-10
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|a NASA Program NSCORT
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|a Non-NASA Center
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|a Antibodies
|2 NLM
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|a Apoproteins
|2 NLM
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|a Carrier Proteins
|2 NLM
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|a Immune Sera
|2 NLM
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7 |
|a Light-Harvesting Protein Complexes
|2 NLM
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650 |
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7 |
|a Photosynthetic Reaction Center Complex Proteins
|2 NLM
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650 |
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7 |
|a Pigments, Biological
|2 NLM
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7 |
|a Chlorophyll
|2 NLM
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|a 1406-65-1
|2 NLM
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|a chlorophyll b
|2 NLM
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7 |
|a 5712ZB110R
|2 NLM
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7 |
|a Chlorophyll A
|2 NLM
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7 |
|a YF5Q9EJC8Y
|2 NLM
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1 |
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|a Armbrust, T
|e verfasserin
|4 aut
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700 |
1 |
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|a Gallegos, G
|e verfasserin
|4 aut
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700 |
1 |
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|a Guikema, J A
|e verfasserin
|4 aut
|
700 |
1 |
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|a Spooner, B S
|e investigator
|4 oth
|
773 |
0 |
8 |
|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 30(1992), 3 vom: 01., Seite 347-56
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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773 |
1 |
8 |
|g volume:30
|g year:1992
|g number:3
|g day:01
|g pages:347-56
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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|a AR
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|d 30
|j 1992
|e 3
|b 01
|h 347-56
|