Characterization of photosystem 1 chlorophyll a/b-binding apoprotein accumulation in developing soybean using type-specific antibodies

The structure and supramolecular assembly of the soybean photosystem 1 (PS 1) chlorophyll a/b-binding antenna (LHC 1) was examined. We identified the subunit composition of LHC 1 in soybean and followed the accumulation of individual subunits during light-induced assembly. We observed four LHC 1 s...

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Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 30(1992), 3 vom: 01., Seite 347-56
1. Verfasser: Henry, R L (VerfasserIn)
Weitere Verfasser: Armbrust, T (BerichterstatterIn), Gallegos, G, Guikema, J A, Spooner, B S
Format: Aufsatz
Sprache:English
Veröffentlicht: 1992
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. NASA Discipline Cell Biology NASA Discipline Developmental Biology NASA Discipline Number 93-10 NASA Program NSCORT Non-NASA Center mehr... Antibodies Apoproteins Carrier Proteins Immune Sera Light-Harvesting Protein Complexes Photosynthetic Reaction Center Complex Proteins Pigments, Biological Chlorophyll 1406-65-1 chlorophyll b 5712ZB110R Chlorophyll A YF5Q9EJC8Y
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100 1 |a Henry, R L  |e verfasserin  |4 aut 
245 1 0 |a Characterization of photosystem 1 chlorophyll a/b-binding apoprotein accumulation in developing soybean using type-specific antibodies 
264 1 |c 1992 
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500 |a Date Revised 13.12.2023 
500 |a published: Print 
500 |a Citation Status MEDLINE 
520 |a The structure and supramolecular assembly of the soybean photosystem 1 (PS 1) chlorophyll a/b-binding antenna (LHC 1) was examined. We identified the subunit composition of LHC 1 in soybean and followed the accumulation of individual subunits during light-induced assembly. We observed four LHC 1 subunits, at 23, 22, 21 and 20.5 kDa, obtained partial sequence information by amino-terminal sequence analysis, and classified the 20.5, 22, and 21 kDa subunits as being encoded by type I, II, and IV chlorophyll a/b binding protein genes, respectively. Antisera against LHC 1 subunits were used to follow the accumulation of individual subunits during the light-initiated transition from etioplast to chloroplast. Several points are noteworthy. First, monospecific antibody against the 22 kDa subunit decorated a 25 kDa peptide in etiolated tissue, which declined during maturation. This decline correlated with the light-induced appearance of mature 22 kDa peptide, suggesting a precursor/product relationship. Second, the same antibody identified a 22 kDa protein in mature corn, but not a larger band in etiolated corn, suggesting that LHC 1 accumulation is regulated differently between species before the onset of chlorophyll biosynthesis. Third, the mature 22 kDa subunit appeared somewhat later than the other LHC 1 peptides during greening, implying that this subunit is less intimately associated with the PS1 core than are the subunits appearing earlier in development 
650 4 |a Comparative Study 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
650 4 |a Research Support, U.S. Gov't, P.H.S. 
650 4 |a NASA Discipline Cell Biology 
650 4 |a NASA Discipline Developmental Biology 
650 4 |a NASA Discipline Number 93-10 
650 4 |a NASA Program NSCORT 
650 4 |a Non-NASA Center 
650 7 |a Antibodies  |2 NLM 
650 7 |a Apoproteins  |2 NLM 
650 7 |a Carrier Proteins  |2 NLM 
650 7 |a Immune Sera  |2 NLM 
650 7 |a Light-Harvesting Protein Complexes  |2 NLM 
650 7 |a Photosynthetic Reaction Center Complex Proteins  |2 NLM 
650 7 |a Pigments, Biological  |2 NLM 
650 7 |a Chlorophyll  |2 NLM 
650 7 |a 1406-65-1  |2 NLM 
650 7 |a chlorophyll b  |2 NLM 
650 7 |a 5712ZB110R  |2 NLM 
650 7 |a Chlorophyll A  |2 NLM 
650 7 |a YF5Q9EJC8Y  |2 NLM 
700 1 |a Armbrust, T  |e verfasserin  |4 aut 
700 1 |a Gallegos, G  |e verfasserin  |4 aut 
700 1 |a Guikema, J A  |e verfasserin  |4 aut 
700 1 |a Spooner, B S  |e investigator  |4 oth 
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