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01000naa a22002652 4500 |
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NLM114122342 |
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DE-627 |
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20231222165254.0 |
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tu |
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231222s2001 xx ||||| 00| ||eng c |
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|a pubmed24n0381.xml
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|a (DE-627)NLM114122342
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|a (NLM)11513540
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Uzel, G
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Detection of intracellular phosphorylated STAT-4 by flow cytometry
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| 264 |
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1 |
|c 2001
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 20.09.2001
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| 500 |
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|a Date Revised 17.11.2005
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a The convergence of innate and adaptive immunity is critical for host defense, allowing for early protection and the generation of specific responses. STAT-4 is at that point of convergence, unifying the IFNalpha and IL-12 pathways. Activation of STAT-4 is crucial to T cell polarization, B cell and NK cell activation, and the control of intracellular pathogens. However, techniques to detect phosphorylated STAT-4 are cumbersome and require many cells. We have developed a flow cytometric detection technique to investigate IL-12 signaling in human peripheral blood mononuclear cells. Using different polyclonal antibodies that recognize either total STAT-4 protein or tyrosine-phosphorylated STAT-4, we can easily detect IL-12 and IFNalpha signaling in PHA/IL-2 blasts derived from peripheral blood lymphocytes. This technique not only allows us to evaluate IL-12 signaling, but it is also less time consuming and labor intensive than alternative methods. Using this flow cytometry-based method, we should be able to detect patients with defects in IL-12 receptor signal transduction, who typically present with disseminated nontuberculous mycobacterial infections
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| 650 |
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4 |
|a Journal Article
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| 650 |
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7 |
|a DNA-Binding Proteins
|2 NLM
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| 650 |
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7 |
|a Interferon-alpha
|2 NLM
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| 650 |
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7 |
|a STAT4 Transcription Factor
|2 NLM
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| 650 |
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7 |
|a Stat4 protein, mouse
|2 NLM
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| 650 |
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7 |
|a Trans-Activators
|2 NLM
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| 650 |
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7 |
|a Interleukin-12
|2 NLM
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| 650 |
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7 |
|a 187348-17-0
|2 NLM
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| 700 |
1 |
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|a Frucht, D M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fleisher, T A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Holland, S M
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 100(2001), 3 vom: 01. Sept., Seite 270-6
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
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| 773 |
1 |
8 |
|g volume:100
|g year:2001
|g number:3
|g day:01
|g month:09
|g pages:270-6
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_11
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| 912 |
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|a GBV_ILN_24
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 100
|j 2001
|e 3
|b 01
|c 09
|h 270-6
|