A comparative study of glutathione and ascorbate metabolism during germination of Pinus pinea L. seeds

The ascorbate and glutathione systems have been studied during the first stages of germination in orthodox seeds of the gymnosperm Pinus pinea L. (pine). The results indicate that remarkable changes in the content and redox balance of these metabolites occur in both the embryo and endosperm; even if...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 52(2001), 361 vom: 12. Aug., Seite 1647-54
1. Verfasser: Tommasi, F (VerfasserIn)
Weitere Verfasser: Paciolla, C, de Pinto, M C, De Gara, L
Format: Aufsatz
Sprache:English
Veröffentlicht: 2001
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't Antioxidants Oxidoreductases EC 1.- Peroxidases EC 1.11.1.- Ascorbate Peroxidases EC 1.11.1.11 mehr... Oxidoreductases Acting on CH-CH Group Donors EC 1.3.- galactonolactone dehydrogenase EC 1.3.2.3 NADH, NADPH Oxidoreductases EC 1.6.- monodehydroascorbate reductase (NADH) EC 1.6.5.4 Glutathione Reductase EC 1.8.1.7 glutathione dehydrogenase (ascorbate) EC 1.8.5.1 Glutathione GAN16C9B8O Ascorbic Acid PQ6CK8PD0R Glutathione Disulfide ULW86O013H Dehydroascorbic Acid Y2Z3ZTP9UM
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245 1 2 |a A comparative study of glutathione and ascorbate metabolism during germination of Pinus pinea L. seeds 
264 1 |c 2001 
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500 |a Date Completed 04.10.2001 
500 |a Date Revised 16.11.2017 
500 |a published: Print 
500 |a Citation Status MEDLINE 
520 |a The ascorbate and glutathione systems have been studied during the first stages of germination in orthodox seeds of the gymnosperm Pinus pinea L. (pine). The results indicate that remarkable changes in the content and redox balance of these metabolites occur in both the embryo and endosperm; even if with different patterns for the two redox pairs. Dry seeds are devoid of the ascorbate reduced form (ASC) and contain only dehydroascorbic acid (DHA). By contrast, glutathione is present both in the reduced (GSH) and in the oxidized (GSSG) forms. During imbibition the increase in ASC seems to be mainly caused by the reactivation of its biosynthesis. On the other hand, the GSH rise occurring during the first 24 h seems to be largely due to GSSG reduction, even if GSH biosynthesis is still active in the seeds. The enzymes of the ascorbate--glutathione cycle also change during germination, but in different ways. ASC peroxidase (EC 1.11.1.11) and glutathione reductase (EC 1.6.4.2) activities progressively rise both in the embryo and in endosperm. These changes are probably required for counteracting production of reactive oxygen species caused by recovery of oxidative metabolism. The two enzymes involved in the ascorbate recycling, ascorbate free radical (AFR) reductase (EC 1.6.5.4) and DHA reductase (EC 1.8.5.1), show different behaviour: the DHA reductase activity decreases, while that of AFR reductase remains unchanged. The relationship between ascorbate and glutathione metabolism and their relevance in the germination of orthodox seeds are also discussed 
650 4 |a Comparative Study 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Antioxidants  |2 NLM 
650 7 |a Oxidoreductases  |2 NLM 
650 7 |a EC 1.-  |2 NLM 
650 7 |a Peroxidases  |2 NLM 
650 7 |a EC 1.11.1.-  |2 NLM 
650 7 |a Ascorbate Peroxidases  |2 NLM 
650 7 |a EC 1.11.1.11  |2 NLM 
650 7 |a Oxidoreductases Acting on CH-CH Group Donors  |2 NLM 
650 7 |a EC 1.3.-  |2 NLM 
650 7 |a galactonolactone dehydrogenase  |2 NLM 
650 7 |a EC 1.3.2.3  |2 NLM 
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650 7 |a EC 1.6.-  |2 NLM 
650 7 |a monodehydroascorbate reductase (NADH)  |2 NLM 
650 7 |a EC 1.6.5.4  |2 NLM 
650 7 |a Glutathione Reductase  |2 NLM 
650 7 |a EC 1.8.1.7  |2 NLM 
650 7 |a glutathione dehydrogenase (ascorbate)  |2 NLM 
650 7 |a EC 1.8.5.1  |2 NLM 
650 7 |a Glutathione  |2 NLM 
650 7 |a GAN16C9B8O  |2 NLM 
650 7 |a Ascorbic Acid  |2 NLM 
650 7 |a PQ6CK8PD0R  |2 NLM 
650 7 |a Glutathione Disulfide  |2 NLM 
650 7 |a ULW86O013H  |2 NLM 
650 7 |a Dehydroascorbic Acid  |2 NLM 
650 7 |a Y2Z3ZTP9UM  |2 NLM 
700 1 |a Paciolla, C  |e verfasserin  |4 aut 
700 1 |a de Pinto, M C  |e verfasserin  |4 aut 
700 1 |a De Gara, L  |e verfasserin  |4 aut 
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