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231222s2001 xx ||||| 00| ||eng c |
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Oliva, A A
|c Jr
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Fluorescence in situ hybridization method for co-localization of mRNA and GEP
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1 |
|c 2001
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 07.12.2001
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|a Date Revised 28.09.2018
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|a published: Print
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|a Citation Status MEDLINE
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|a Co-localization studies using green fluorescent protein (GFP) and fluorescence immunohistochemistry have become commonplace. However, co-localization studies using GFP and mRNA in situ hybridization are rare, in large part because typical in situ hybridization reaction conditions often lead to the loss of GFP fluorescence. Here, we describe a new fluorescence mRNA in situ hybridization protocol using cRNA riboprobes that leaves GFP fluorescence intact. This protocol is based on a urea-based hybridization buffer and the Tyramide Signal Amplification system. This protocol should provide researchers engaged in the use of GFP with a solid starting point for adapting their own in situ hybridization protocols
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| 650 |
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, P.H.S.
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| 650 |
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4 |
|a Technical Report
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4 |
|a Journal Article
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| 650 |
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7 |
|a Indicators and Reagents
|2 NLM
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| 650 |
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7 |
|a Luminescent Proteins
|2 NLM
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| 650 |
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7 |
|a RNA, Messenger
|2 NLM
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| 650 |
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|a Green Fluorescent Proteins
|2 NLM
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| 650 |
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7 |
|a 147336-22-9
|2 NLM
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| 700 |
1 |
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|a Swann, J W
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 31(2001), 1 vom: 12. Juli, Seite 74-6, 78-81
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
1 |
8 |
|g volume:31
|g year:2001
|g number:1
|g day:12
|g month:07
|g pages:74-6, 78-81
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|d 31
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|h 74-6, 78-81
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