Sulphur nutrition affects delivery and metabolism of S in developing endosperms of wheat
Experiments were conducted to investigate the effect of S nutrition and availability on the forms of S and N in the endosperm cavity and endosperm of wheat, and on the capacity of the endosperm to utilize those compounds for the synthesis of proteins. Plants were grown in solution culture with 2 mM...
| Publié dans: | Journal of experimental botany. - 1985. - 52(2001), 360 vom: 16. Juli, Seite 1519-26 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
2001
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| Accès à la collection: | Journal of experimental botany |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Sulfates Glutamic Acid 3KX376GY7L Sulfur 70FD1KFU70 Adenosine Triphosphate 8L70Q75FXE Cysteine Synthase plus... |
| Résumé: | Experiments were conducted to investigate the effect of S nutrition and availability on the forms of S and N in the endosperm cavity and endosperm of wheat, and on the capacity of the endosperm to utilize those compounds for the synthesis of proteins. Plants were grown in solution culture with 2 mM N and either 200 microM S (high-S) or 50 microM S (low-S) and all nutrients were withdrawn at various times from booting until 8 d post-anthesis. Sulphate was the major form of soluble S in the endosperm cavity and endosperm of high-S plants during the time of rapid grain development. By contrast, glutathione (GSH) was the major form of soluble S in the endosperm cavity and in the endosperm in low-S plants. Crude extracts of endosperm tissue from both high-S and low-S plants supported (i) the hydrolysis of GSH to gamma-glutamyl cysteine and glycine, and of gamma-glutamyl cysteine to glutamate and cysteine, and (ii) sulphate-dependent PPi-ATP exchange and the sulphydration of O-acetylserine catalysed by ATP sulphurylase and cysteine synthase, respectively. High-S nutrition enhanced the in vitro rates of ATP sulphurylase and cysteine synthase |
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| Description: | Date Completed 04.10.2001 Date Revised 13.05.2019 published: Print Citation Status MEDLINE |
| ISSN: | 1460-2431 |