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NLM113361505 |
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DE-627 |
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20231222163708.0 |
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tu |
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231222s2001 xx ||||| 00| ||eng c |
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|a pubmed24n0378.xml
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|a (DE-627)NLM113361505
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|a (NLM)11432914
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Gullner, G
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Enhanced tolerance of transgenic poplar plants overexpressing gamma-glutamylcysteine synthetase towards chloroacetanilide herbicides
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| 264 |
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1 |
|c 2001
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 30.08.2001
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|a Date Revised 13.05.2019
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|a published: Print
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|a Citation Status MEDLINE
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|a A wild-type poplar hybrid and two transgenic clones overexpressing a bacterial gamma-glutamylcysteine synthetase in the cytosol or in the chloroplasts were exposed to the chloroacetanilide herbicides acetochlor and metolachlor dispersed in the soil. The transformed poplars contained higher gamma-glutamylcysteine and glutathione (GSH) levels than wild-type plants and therefore it was supposed that they would have an elevated tolerance towards these herbicides, which are detoxified in GSH-dependent reactions. Phenotypically, the transgenic and wild-type plants did not differ. The growth and the biomass of all poplar lines were markedly reduced by the two chloroacetanilide herbicides. However, the decrease of shoot and root fresh weights caused by the herbicides was significantly smaller in the transgenic than in wild-type plants. In addition, the growth rate of poplars transformed in the cytosol was reduced to a significantly lesser extent than that of wild-type plants following herbicide treatments. The effects of the two herbicides were similar. Herbicide exposures markedly increased the levels of gamma-glutamylcysteine and GSH in leaves of each poplar line. The increase in the foliar amounts of these thiols was stronger in the transgenic lines than in the wild type, particularly in the upper leaves. Considerable GST activities were detected in leaves of all poplar plants. Exposure of poplars to chloroacetanilide herbicides resulted in a marked induction of GST activity in upper leaf positions but not in middle and lower leaves. The extent of enzyme induction did not differ significantly between transgenic and wild-type poplars. Although the results show that the transgenic poplar lines are good candidates for phytoremediation purposes, the further improvement of their detoxification capacity, preferably by transformation using genes encoding herbicide-specific GST isoenzymes, seems to be the most promising way to obtain plants suitable for practical application
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| 650 |
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Acetamides
|2 NLM
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| 650 |
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|a Dipeptides
|2 NLM
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| 650 |
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7 |
|a Herbicides
|2 NLM
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| 650 |
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7 |
|a Soil
|2 NLM
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| 650 |
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|a Sulfhydryl Compounds
|2 NLM
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| 650 |
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|a Toluidines
|2 NLM
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| 650 |
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|a acetochlor
|2 NLM
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| 650 |
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7 |
|a 8L08WMO94K
|2 NLM
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| 650 |
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7 |
|a Peptide Synthases
|2 NLM
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| 650 |
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7 |
|a EC 6.3.2.-
|2 NLM
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| 650 |
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7 |
|a gamma-glutamylhistamine synthetase
|2 NLM
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| 650 |
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7 |
|a EC 6.3.2.-
|2 NLM
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| 650 |
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7 |
|a gamma-glutamylcysteine
|2 NLM
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| 650 |
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7 |
|a M984VJS48P
|2 NLM
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| 650 |
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7 |
|a metolachlor
|2 NLM
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| 650 |
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7 |
|a X0I01K05X2
|2 NLM
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| 700 |
1 |
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|a Kömives, T
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rennenberg, H
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 52(2001), 358 vom: 01. Mai, Seite 971-9
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
1 |
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|g volume:52
|g year:2001
|g number:358
|g day:01
|g month:05
|g pages:971-9
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 52
|j 2001
|e 358
|b 01
|c 05
|h 971-9
|