Stable luciferase reporter cell lines for signal transduction pathway readout using GAL4 fusion transactivators

While GAL4 fusion activators have been widely used for dissecting signal transduction pathways in transient assays, there has been surprisingly little reported on utilizing cell lines with stably integrated fusion activators. To avoid problems with the efficiency and reproducibility inherent to tran...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 30(2001), 5 vom: 01. Mai, Seite 1134-8, 1140
1. Verfasser: Hexdall, L (VerfasserIn)
Weitere Verfasser: Zheng, C F
Format: Aufsatz
Sprache:English
Veröffentlicht: 2001
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Cyclic AMP Response Element-Binding Protein DNA-Binding Proteins Fungal Proteins GAL4 protein, S cerevisiae Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors Epidermal Growth Factor mehr... 62229-50-9 DNA 9007-49-2 Luciferases EC 1.13.12.- Protein Serine-Threonine Kinases EC 2.7.11.1 Cyclic AMP-Dependent Protein Kinases EC 2.7.11.11 Mitogen-Activated Protein Kinases EC 2.7.11.24 MAP Kinase Kinase 1 EC 2.7.12.2 MAP2K1 protein, human Mitogen-Activated Protein Kinase Kinases Tetradecanoylphorbol Acetate NI40JAQ945