S-Gal : an autoclavable dye for color selection of cloned DNA inserts
Blue/white selection is the standard method for detecting a cloned DNA fragment. In the absence of an insert, uninterrupted expression of the vector-encoded alpha-complement of beta-galactosidase (beta-gal), results in the hydrolysis of X-gal (5-bromo-4-chloro-3-indolyl-beta-D-galactoside) and the s...
| Publié dans: | BioTechniques. - 1991. - 30(2001), 5 vom: 01. Mai, Seite 1142-7 |
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| Format: | Article |
| Langue: | English |
| Publié: |
2001
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Coloring Agents DNA, Recombinant Galactosides Umbelliferones cyclohexenoesculetin-beta-galactoside beta-Galactosidase EC 3.2.1.23 |