Use of an ALFexpress DNA sequencer to analyze protein-nucleic acid interactions by band shift assay
Gel retardation analysis, or band shift assay, is technically the simplest method to investigate protein-nucleic acid interactions. In this report, we describe a nonradioactive band shift assay using a fluorescent DNA target and an ALFexpress automatic DNA sequencer in place of the current method th...
| Publié dans: | BioTechniques. - 1991. - 30(2001), 5 vom: 01. Mai, Seite 1044-8, 1050-1 |
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| Auteur principal: | |
| Autres auteurs: | , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2001
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Bacterial Proteins DNA-Binding Proteins Escherichia coli Proteins Lac Repressors Repressor Proteins AmpR protein, Bacteria 125267-46-1 DNA plus... |