Isothermal amplification and multimerization of DNA by Bst DNA polymerase

We have demonstrated the isothermal in vitro amplification and multimerization of several different linear DNA targets using only two primers and the strongly strand-displacing exonuclease-negative Bst DNA polymerase. This reaction has been termed linear target isothermal multimerization and amplifi...

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Détails bibliographiques
Publié dans:BioTechniques. - 1991. - 30(2001), 4 vom: 01. Apr., Seite 852-6, 858, 860 passim
Auteur principal: Hafner, G J (Auteur)
Autres auteurs: Yang, I C, Wolter, L C, Stafford, M R, Giffard, P M
Format: Article
Langue:English
Publié: 2001
Accès à la collection:BioTechniques
Sujets:Journal Article Research Support, Non-U.S. Gov't DNA Primers DNA, Bacterial DNA, Circular Indicators and Reagents Streptavidin 9013-20-1 DNA Polymerase I EC 2.7.7.7