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231222s2001 xx ||||| 00| ||eng c |
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|a pubmed25n0373.xml
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|a (DE-627)NLM111926971
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|a (NLM)11283182
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Hultine, K R
|e verfasserin
|4 aut
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|a A comparison of three methods for determining the stomatal density of pine needles
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|c 2001
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 30.08.2001
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|a Date Revised 16.11.2017
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|a published: Print
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|a Citation Status MEDLINE
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|a Alternative methods were compared for determining the stomatal density of needles from two pine species. Densities estimated from air-dried, whole needles using a binocular dissecting scope were compared to densities estimated from vacuum-dried, intact needles using a scanning electron microscope and expanded peels (or macerated cuticles) using a compound light microscope. Differences among methods were expected from two sources: (1) expansion and shrinkage as a function of water content, and (2) differences in geometry of the measured surface. Estimates from the dissecting scope were similar to those from scanning electron microscopy (t=0.509, n=21, P:=0.62), presumably because both used dried, but otherwise intact whole needles. Light microscopy estimates, however, were lower than dissecting scope estimates (t=-2.307, n=13, P:=0.04). After adjusting for expansion due to hydration and changes in needle geometry, differences disappeared (t=-1.205, n=13, P:=0.25). These results are an important consideration for researchers reconstructing palaeo-atmospheric conditions and assessing plant response to environmental change
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|a Comparative Study
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|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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|a Water
|2 NLM
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| 650 |
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|a 059QF0KO0R
|2 NLM
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| 700 |
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|a Marshall, J D
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 52(2001), 355 vom: 02. Feb., Seite 369-73
|w (DE-627)NLM098182706
|x 0022-0957
|7 nnns
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| 773 |
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|g volume:52
|g year:2001
|g number:355
|g day:02
|g month:02
|g pages:369-73
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|a AR
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|d 52
|j 2001
|e 355
|b 02
|c 02
|h 369-73
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