Rapid N transport to pods and seeds in N-deficient soybean plants

Non-nodulated soybean (Glycine max (L.) Merr.) plants were cultivated hydroponically under N-sufficient (5 mM NaNO(3)) or N-deficient (0.5 mM NaNO(3)) conditions. (13)N- or (15)N- labelled nitrate was fed to the cut end of the stems, and the accumulation of nitrate-derived N in the pods, nodes and s...

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Détails bibliographiques
Publié dans:Journal of experimental botany. - 1985. - 52(2001), 355 vom: 02. Feb., Seite 277-83
Auteur principal: Ohtake, N (Auteur)
Autres auteurs: Sato, T, Fujikake, H, Sueyoshi, K, Ohyama, T, Ishioka, N S, Watanabe, S, Osa, A, Sekine, T, Matsuhashi, S, Ito, T, Mizuniwa, C, Kume, T, Hashimoto, S, Uchida, H, Tsuji, A
Format: Article
Langue:English
Publié: 2001
Accès à la collection:Journal of experimental botany
Sujets:Comparative Study Journal Article Amino Acids Nitrates Nitrogen Radioisotopes Nitrogen N762921K75
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520 |a Non-nodulated soybean (Glycine max (L.) Merr.) plants were cultivated hydroponically under N-sufficient (5 mM NaNO(3)) or N-deficient (0.5 mM NaNO(3)) conditions. (13)N- or (15)N- labelled nitrate was fed to the cut end of the stems, and the accumulation of nitrate-derived N in the pods, nodes and stems was compared. Real-time images of (13)N distribution in stems, petioles and pods were obtained using a Positron Emitting Tracer Imaging System for a period of 40 min. The results indicated that the radioactivity in the pods of N-deficient plants was about 10 times higher than that of N-sufficient plants, although radioactivity in the stems and nodes of N-deficient versus N-sufficient plants was not different. A similar result was obtained by supplying (15)NO(3) to cut soybean shoots for 1 h. The fact that the N translocation into the pods from NO(3) fed to the stem base was much faster in N-deficient plants may be due to the strong sink activity of the pods in N-deficient plants. Alternatively, the redistribution of N from the leaves to the pods via the phloem may be accelerated in N-deficient plants. The temporal accumulation of (13)NO(3) in nodes was suggested in both N-sufficient and N-deficient plants. In one (13)NO(3) pulse-chase experiment, radioactivity in the stem declined rapidly after transferring the shoot from the (13)NO(3) solution to non-labelled NO(3); in contrast, the radioactivity in the node declined minimally during the same time period 
650 4 |a Comparative Study 
650 4 |a Journal Article 
650 7 |a Amino Acids  |2 NLM 
650 7 |a Nitrates  |2 NLM 
650 7 |a Nitrogen Radioisotopes  |2 NLM 
650 7 |a Nitrogen  |2 NLM 
650 7 |a N762921K75  |2 NLM 
700 1 |a Sato, T  |e verfasserin  |4 aut 
700 1 |a Fujikake, H  |e verfasserin  |4 aut 
700 1 |a Sueyoshi, K  |e verfasserin  |4 aut 
700 1 |a Ohyama, T  |e verfasserin  |4 aut 
700 1 |a Ishioka, N S  |e verfasserin  |4 aut 
700 1 |a Watanabe, S  |e verfasserin  |4 aut 
700 1 |a Osa, A  |e verfasserin  |4 aut 
700 1 |a Sekine, T  |e verfasserin  |4 aut 
700 1 |a Matsuhashi, S  |e verfasserin  |4 aut 
700 1 |a Ito, T  |e verfasserin  |4 aut 
700 1 |a Mizuniwa, C  |e verfasserin  |4 aut 
700 1 |a Kume, T  |e verfasserin  |4 aut 
700 1 |a Hashimoto, S  |e verfasserin  |4 aut 
700 1 |a Uchida, H  |e verfasserin  |4 aut 
700 1 |a Tsuji, A  |e verfasserin  |4 aut 
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