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|a eng
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1 |
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|a Leung, Y F
|e verfasserin
|4 aut
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|a High-throughput conformation-sensitive gel electrophoresis for discovery of SNPs
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|c 2001
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|2 rdacarrier
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|a Date Completed 24.05.2001
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|a Date Revised 28.09.2018
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a High-throughput screening for single nucleotide polymorphisms (SNPs) or mutations can be achieved by inexpensive technologies. We modified the original protocols of conformation-sensitive gel electrophoresis (CSGE) to increase throughput several fold to 1.3 samples/min, which is about five times faster than denaturing high-performance liquid chromatography (DHPLC). The modifications include decreasing the gel thickness, increasing the number of lanes to 96, and increasing the number of samples per lane to seven. This high-throughput CSGE method is fast, robust, and as simple as the original protocols. Together with a two-stage strategy for screening homozygotes and the replacement of ethidium bromide with SYBR Gold DNA dye staining, this protocol is a reliable and cost-effective alternative for laboratories that require high-throughput screening
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Tam, P O
|e verfasserin
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| 700 |
1 |
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|a Tong, W C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Baum, L
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Choy, K W
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|4 aut
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| 700 |
1 |
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|a Lam, D S
|e verfasserin
|4 aut
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| 700 |
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|a Pang, C P
|e verfasserin
|4 aut
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|i Enthalten in
|t BioTechniques
|d 1993
|g 30(2001), 2 vom: 09. Feb., Seite 334-5, 338-40
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|x 0736-6205
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