Quantitative detection of Chlamydia spp. by fluorescent PCR in the LightCycler
Quantitative detection of intracellular bacteria of the genus Chlamydia by the standard cell culture method is cumbersome and operator dependent. As an alternative, we adapted hot-start PCR to the glass capillary quantitative PCR format of the LightCycler. The optimized PCR was consistently more eff...
| Publié dans: | BioTechniques. - 1991. - 30(2001), 1 vom: 22. Jan., Seite 150-7 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2001
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Bacterial Outer Membrane Proteins Benzothiazoles DNA, Bacterial Diamines Fluorescent Dyes Organic Chemicals Porins plus... |