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20250202114322.0 |
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tu |
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231222s2001 xx ||||| 00| ||eng c |
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|a pubmed25n0371.xml
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|a (DE-627)NLM111083516
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|a (NLM)11196302
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Wedemayer, G J
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Conversion of capillary electrophoresis microchip genotyping data for analysis with Genetic Profiler software
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1 |
|c 2001
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 29.03.2001
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|a Date Revised 14.11.2007
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|a published: Print
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|a Citation Status MEDLINE
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|a The collection and conversion of 4-color fluorescent genotyping data from capillary array electrophoresis microchip devices and its conversion to a format easily and rapidly analyzed by Genetic Profiler genotyping software is presented. Microchip fluorescence intensity data are acquired and stored as 4-color tab-delimited text. These files are converted to electrophoretic signal data (ESD) files using a utility program (TEXT-to-ESD) written in C. TEXT-to-ESD generates an ESD file by converting text data to binary data and then appending a 632-byte ESD-file trailer. Up to 96 ESD files are then assembled into a run folder and imported into Genetic Profiler, where data are reduced to 4-color electropherograms and analyzed. In this manner, DNA fragment sizing data acquired with our high-speed electrophoretic microchip devices can be rapidly analyzed using robust commercial software. Additionally, the conversion program allows sizing of data with Genetic Profiler that have been preprocessed using other third-party software, such as BaseFinder
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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4 |
|a Research Support, U.S. Gov't, P.H.S.
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| 650 |
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7 |
|a DNA
|2 NLM
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| 650 |
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7 |
|a 9007-49-2
|2 NLM
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| 700 |
1 |
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|a Medintz, I
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wong, W W
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Scherer, J R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mathies, R A
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 30(2001), 1 vom: 22. Jan., Seite 122-8
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
1 |
8 |
|g volume:30
|g year:2001
|g number:1
|g day:22
|g month:01
|g pages:122-8
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|d 30
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