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231222s2001 xx ||||| 00| ||eng c |
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Madden, M
|e verfasserin
|4 aut
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| 245 |
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|a Hybridization screening of very short PCR products for paleoepidemiological studies of Chagas' disease
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|c 2001
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 29.03.2001
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|a Date Revised 28.09.2018
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|a published: Print
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|a Citation Status MEDLINE
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|a Single strands of very short PCR products can be covalently immobilized to a slide and then easily detected by probe hybridization. In this work, the PCR product was a 70-nucleotide segment of ancient DNA, representing a portion of repeat mini-circle DNA from the kinetoplast of Trypanosoma cruzi, the infectious agent of Chagas' disease (American Trypanosomiasis). The target segment was initially established to be present in soft tissue samples taken from four "naturally" mummified Andean bodies using PCR followed by cloning and sequencing. Hybridization screening of the covalently immobilized PCR products positively identified products from 25 of 27 specimens of different tissues from these four mummies. The method appears to be ideal for the purpose of screening a large number of specimens when the target PCR product is very short
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Technical Report
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|a Journal Article
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| 650 |
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|a DNA, Kinetoplast
|2 NLM
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| 650 |
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7 |
|a RNA Probes
|2 NLM
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| 700 |
1 |
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|a Salo, W L
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Streitz, J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Aufderheide, A C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fornaciari, G
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jaramillo, C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Vallejo, G A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yockteng, R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Arriaza, B
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cárdenas-Arroyo, F
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Guhl, F
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 30(2001), 1 vom: 22. Jan., Seite 102-4, 106, 108-9
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
1 |
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|g volume:30
|g year:2001
|g number:1
|g day:22
|g month:01
|g pages:102-4, 106, 108-9
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